Do not surround your terms in double-quotes () in this field.Two hundred Amplified Fragment Length Polymorphism (AFLP)markers were identified and utilized in the analysis.Relatedness amongaccessions was estimated by analyzing the AFLP data using the Dice coefficientof similarity and UPGMA methods.The molecular analysis revealed relativelylimited genetic diversity within and between sites.
Genotypes collected in agiven region often displayed molecular marker variability similar to thatobserved over the entire sampled area. However, a subset of 14 genotypes derivedfrom seed collected from New Ireland island differed from genotypes collected onNew Guinea island. Estimates of genetic diversity-based similarity valuescalculated from the AFLP data indicated a moderate level of diversity (0.767mean coefficient of similarity) across all plant materials analyzed. Threemethods of selection were evaluated for their efficacy in capturing themolecular marker diversity within the plant materials in the form of a subset.They were random, stratified-random (geographic based), and marker-assistedselection (MAS). A Maximally Diverse Subset (MDS) of12 genotypes capturing 92 of the molecular marker diversity was identified. They were placed on your computer when you launched this website. If coincident patterns are detected for a given set of samples using either type of marker then the results obtained from both are directly comparable. A Comparison of RAPD Versus Microsatellite DNA Markers in. OSU. Microsatellite DNA Markers in Shorea platyclados (Dipterocarpaceae. Identification of rice hybrids using microsatellite and RAPD markers. Characterization and comparison of microsatellite markers derived. A comparison of RAPD and ISSR markers reveals genetic diversity. Two key processes. Heredity 83: 260-270. Ewens, W.J. 1990: The minimum viable population size as a. Comparison between RAPD and SSR molecular markers in detecting. Comparison of RAPD, RFLP, AFLP and SSR markers for. SciELO. Comparison of RAPD, ISSR, and DAMD Markers for Genetic Diversity. Comparison of RAPD, ISSR, and AFLP Molecular Markers to Reveal. Random Amplified Polymorphic DNA (RAPD) Sequences as Markers. Random amplified polymorphic DNA (RAPD) markers and. CiteSeerX. INTRODUCTION. Advances in molecular biology techniques have provided the basis for uncovering virtu-. A Comparison of RAPD Versus Microsatellite DNA Markers in Population Studies of the Massasauga Rattlesnake S. C. Lougheed, H. L. Gibbs, K. A. Prior, and P. J. Weatherhead We compared genetic differentiation among populations of the threatened massasauga rattlesnake (Sistrurus c. Analyses of molecular variance (AMOVA) and GST and FST analyses indicated that levels of among-population differentiation between regional populations (100 km) were comparable for both markers. However, microsatellites were superior in population assignment tests and at discerning fine-scale genetic differentiation between subpopulations separated by tens of kilometers. These results argue that both types of markers are suitable for defining broad-scale genetic structures in snake populations and can provide important inputs into conservation initiatives of focal taxa. However, our analyses suggest that microsatellites are better for detecting structure at limited spatial scales. From the Department of Biology, Queens University, Kingston, Ontario, Canada K7L 3N6 ( Lougheed), Department of Biology, McMaster University, Hamilton, Ontario, Canada (Gibbs), Endangered Species Division, Canadian Wildlife Service, Hull, Quebec, Canada (Prior), and Department of Biology, Carleton University, Ottawa, Ontario, Canada (Weatherhead). Two anonymous reviewers provided comments that improved the manuscript immeasurably. Obviously these markers are not equally applicable to all situations and their relative merits and disadvantages have been summarized elsewhere (cf. Parker et al. 1998). For example, the technical ease and lower costs of developing RAPDs may make them better for some applications ( Karp et al. However, because RAPDs exhibit dominance, their use in testing for departures from HardyWeinberg equilibrium or for estimating inbreeding is precluded ( Hadrys et al. Ultimately it would be valuable to determine if microsatellites and RAPDs are equally effective at detecting patterns of differentiation that develop over similar time scales.
0 Comments
Leave a Reply. |
Details
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |